6A3E

MVM NES mutant Nm15 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	105.688, 105.688, 303.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.310 - 2.700
R-factor	0.2053
Rwork	0.203
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hat
RMSD bond length	0.006
RMSD bond angle	1.025
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.209	0.104
Rmeas	0.209	0.109
Rpim	0.077	0.032	0.694
Number of reflections	49549	2771	2442
<I/σ(I)>	2.7
Completeness [%]	100.0	99.9	100
Redundancy	12	11.6	10.9
CC(1/2)		0.994	0.377

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% PEG3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH6.6