6A3B
MVM NES mutant Nm13 in complex with CRM1-Ran-RanBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 106.362, 106.362, 303.858 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.510 |
R-factor | 0.2146 |
Rwork | 0.213 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hat |
RMSD bond length | 0.008 |
RMSD bond angle | 1.183 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.165 | 0.085 | |
Rmeas | 0.172 | 0.089 | |
Rpim | 0.061 | 0.024 | |
Total number of observations | 857808 | ||
Number of reflections | 61540 | 3397 | 3024 |
<I/σ(I)> | 4.2 | ||
Completeness [%] | 100.0 | 99.5 | 99.6 |
Redundancy | 13.9 | 13.1 | 9.3 |
CC(1/2) | 0.997 | 0.413 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 18% PEG3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH6.6 |