6A3B

MVM NES mutant Nm13 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	106.362, 106.362, 303.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.510
R-factor	0.2146
Rwork	0.213
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hat
RMSD bond length	0.008
RMSD bond angle	1.183
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.165	0.085
Rmeas	0.172	0.089
Rpim	0.061	0.024
Total number of observations	857808
Number of reflections	61540	3397	3024
<I/σ(I)>	4.2
Completeness [%]	100.0	99.5	99.6
Redundancy	13.9	13.1	9.3
CC(1/2)		0.997	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	18% PEG3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH6.6