6A38

MVM NS2 NES in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	105.438, 105.438, 303.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.690
R-factor	0.22
Rwork	0.218
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hat
RMSD bond length	0.007
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.000	2.750
High resolution limit [Å]	2.690	7.320	2.700
Rmerge	0.169	0.070
Rmeas	0.175	0.073
Rpim	0.063	0.020	0.897
Number of reflections	48581	2710	2361
<I/σ(I)>	3.5
Completeness [%]	100.0	99.6	100
Redundancy	14.3	13	14.6
CC(1/2)		0.998	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	18% PEG3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH6.6