6A2I

Architectural roles of Cren7 in folding crenarchaeal chromatin filament

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	114.963, 29.940, 79.287
Unit cell angles	90.00, 123.88, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.2263
Rwork	0.225
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lwh
RMSD bond length	0.003
RMSD bond angle	0.797
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.400	2.400
Rmerge	0.056	0.484
Number of reflections	9361
<I/σ(I)>	54.8
Completeness [%]	99.4
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.05M KH2PO4, 20% PEG 8000