6A2F
Crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-15 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.240, 78.300, 154.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.500 |
| R-factor | 0.19041 |
| Rwork | 0.188 |
| R-free | 0.23847 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rcq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.098 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | 0.174 |
| Number of reflections | 28844 | 2680 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 90.4 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 4%(v/v) Tacsimate pH 4.0, 12%(w/v) polyethylene glycol 3350 |
