6A2E
Apo structure of the Kdo hydroxylase KdoO, a non-heme Fe(II) alphaketoglutarate dependent dioxygenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.896, 59.541, 116.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.605 - 1.939 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.20520 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.767 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.939 | 5.260 | 1.940 |
Rmerge | 0.097 | 0.041 | 0.281 |
Number of reflections | 24341 | 1367 | 1038 |
<I/σ(I)> | 8.5 | 5.16 | |
Completeness [%] | 99.3 | 99.7 | 87.1 |
Redundancy | 7 | 6.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 288 | 0.1M Sodium acetate, 200mM Lithium sulfate, 50% v/v PEG 400 |