6A20
Crystal Structure of auto-inhibited Kinesin-3 KIF13B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 32 |
Unit cell lengths | 75.070, 75.070, 91.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.639 - 2.400 |
R-factor | 0.1962 |
Rwork | 0.193 |
R-free | 0.24880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zbs |
RMSD bond length | 0.002 |
RMSD bond angle | 0.602 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.097 | 0.615 |
Number of reflections | 22223 | 3271 |
<I/σ(I)> | 8 | |
Completeness [%] | 98.3 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 289 | 0.2M proline, 0.1M HEPES, pH 7.5, 14% (w/v) PEG 3350 |