6A1U

Charcot-Leyden crystal protein/Galectin-10 variant E33D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 65 2 2
Unit cell lengths	48.774, 48.774, 259.354
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.561 - 1.620
R-factor	0.1731
Rwork	0.171
R-free	0.19280
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.561	1.650
High resolution limit [Å]	1.620	1.620
Number of reflections	24648
<I/σ(I)>	24.4
Completeness [%]	99.9
Redundancy	17.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bistris