6A1T

Charcot-Leyden crystal protein/Galectin-10 variant E33A with lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 65 2 2
Unit cell lengths	48.944, 48.944, 262.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.652 - 1.970
R-factor	0.1924
Rwork	0.188
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.652	2.020
High resolution limit [Å]	1.970	1.970
Number of reflections	14247
<I/σ(I)>	12.9
Completeness [%]	99.8
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bistris