6A1O

Crystal structures of disordered Z-type helices

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-10-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	31.112, 31.112, 43.044
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.816 - 2.490
R-factor	0.2745
Rwork	0.280
R-free	0.29940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d53
RMSD bond length	0.009
RMSD bond angle	1.236
Data reduction software	XDS (GPLv2)
Data scaling software	XDS (GPLv2)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	16.816	16.816	2.640
High resolution limit [Å]	2.490	6.890	2.490
Rmerge	0.084	0.056	0.171
Rmeas	0.096	0.065	0.194
Total number of observations	6944
Number of reflections	1584	73	257
<I/σ(I)>	9.95	13.12	6.24
Completeness [%]	97.1	92.4	97
Redundancy	4.384	4.37	4.397
CC(1/2)	0.996	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1mM DNA, 50mM sodium cacodylate trihydrate buffer, 50mM CaCl2, 50% MPD.