6A10
Crystal structure of hen egg white lysozyme crystallized by ammonium sulfate
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 41.399, 57.971, 57.618 |
| 格子定数 [度] | 115.97, 105.20, 98.81 |
精密化法
| 残基 | 19.295 - 1.130 |
| R因子 | 0.164 |
| Rwork | 0.163 |
| R-free | 0.17610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wld |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.365 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 1.150 |
| 分解能 [Å] (高) | 1.130 | 3.070 | 1.130 |
| Rmerge_l_obs | 0.042 | 0.022 | 0.359 |
| Rmeas | 0.060 | 0.032 | 0.508 |
| Rpim | 0.042 | 0.022 | 0.359 |
| Total number of observations | 293247 | ||
| 独立反射数 | 151134 | 5732 | 7388 |
| <I/σ(I)> | 12.8 | ||
| 完全性 [%] | 91.4 | 69.4 | 89.1 |
| 冗長性 | 1.9 | 1.9 | 1.9 |
| CC(1/2) | 0.995 | 0.774 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.2M ammonium sulfate, 6%(v/v) 1,4-dioxane |
