6A0L
Cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from Arthrobacter globiformis, complex with maltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-05 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.000, 115.688, 182.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.100 - 2.100 |
| R-factor | 0.25562 |
| Rwork | 0.252 |
| R-free | 0.32341 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zxg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.777 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.100 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.141 | 0.552 |
| Number of reflections | 26693 | 25316 |
| <I/σ(I)> | 0.115 | 0.022 |
| Completeness [%] | 88.6 | 96.7 |
| Redundancy | 4 | 4.6 |
| CC(1/2) | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1 M Sodium citrte, 0.22 M Ammonium sulfate, 30% (w/v) PEG 4000 |
