6A0K
Cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from Arthrobacter globiformis, complex with panose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.293, 180.225, 62.926 |
Unit cell angles | 90.00, 111.60, 90.00 |
Refinement procedure
Resolution | 90.100 - 1.940 |
R-factor | 0.18946 |
Rwork | 0.188 |
R-free | 0.22297 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xzg |
RMSD bond length | 0.020 |
RMSD bond angle | 1.983 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.100 | 1.970 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.101 | 0.352 |
Number of reflections | 72961 | 69198 |
<I/σ(I)> | 0.137 | 0.024 |
Completeness [%] | 99.5 | 93 |
Redundancy | 3.3 | 3 |
CC(1/2) | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1 M Sodium citrate, 0.22 M Ammonium sulfate, 30% (w/v) PEG 4000 |