6A0J

Cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from Arthrobacter globiformis, complex with Cyclic alpha-maltosyl-(1-->6)-maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-18
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	72.703, 72.703, 209.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.670 - 1.600
R-factor	0.16347
Rwork	0.162
R-free	0.18958
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xzg
RMSD bond length	0.027
RMSD bond angle	2.337
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.700	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.080	0.433
Number of reflections	73605	69714
<I/σ(I)>	0.295	0.06
Completeness [%]	98.2	98.5
Redundancy	13.6	14
CC(1/2)		0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.3	293	0.1 M Glycine-NaOH, 0.2 M Lithium sulfate, 0.8 M Sodium/potassium tartrate, 5 mM Cyclic alpha-maltosyl-(1-->6)-maltose