6ZZJ

Crystal structure of the catalytic domain of Corynebacterium glutamicum acetyltransferase AceF (E2p) in complex with oxidized CoA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.980110
Spacegroup name	P 63 2 2
Unit cell lengths	73.739, 73.739, 192.186
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.050 - 1.350
R-factor	0.1691
Rwork	0.168
R-free	0.19410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zzi
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	autoPROC
Data scaling software	autoPROC (1.0.5)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.050	1.370
High resolution limit [Å]	1.350	1.350
Rpim	0.020	0.545
Number of reflections	68504	3111
<I/σ(I)>	16.1	1.1
Completeness [%]	99.6	92.8
Redundancy	25	21.2
CC(1/2)	0.998	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M Hepes-Na pH 7.5, 1.56 M tri-sodium citrate