6ZZG
MB_CRS6-1 bound to CrSAS-6_N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-23 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 164.451, 347.187, 94.851 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.400 - 2.930 |
R-factor | 0.2228 |
Rwork | 0.221 |
R-free | 0.25380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q0y |
RMSD bond length | 0.003 |
RMSD bond angle | 0.782 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 3.070 |
High resolution limit [Å] | 2.930 | 2.930 |
Number of reflections | 273558 | 9570 |
<I/σ(I)> | 5.98 | |
Completeness [%] | 99.5 | |
Redundancy | 4.5 | |
CC(1/2) | 0.998 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.3 M Sodium acetate trihydrate, 10%w/vPEG8000, 10%w/v PEG 1000, 4% v/v 1,1,1,3,3,3-Hexafluoro-2-propanol, 0.1 M Tris pH 8.5 |