6ZYV
Structure of a Plasmodium PIR protein ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97166 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.170, 63.608, 199.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.840 - 2.150 |
R-factor | 0.2129 |
Rwork | 0.212 |
R-free | 0.23660 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.840 | 2.220 |
High resolution limit [Å] | 2.150 | 2.150 |
Rpim | 0.040 | 0.632 |
Number of reflections | 42523 | 3621 |
<I/σ(I)> | 4.8 | 1.3 |
Completeness [%] | 99.8 | |
Redundancy | 1.9 | |
CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M HEPES pH 6.5 45% w/v Poly(acrylic acid sodium salt) 2100 |