6ZYK
Non-heme monooxygenase, ThoJ-Ni complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 80.753, 80.753, 129.714 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.860 - 2.550 |
| R-factor | 0.2277 |
| Rwork | 0.227 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ncj |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.756 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | Phaser-MR |
| Refinement software | PHENIX ((1.18.2_3874)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.560 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmeas | 0.120 | 0.954 |
| Number of reflections | 14610 | 1414 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 150 mM Sodium tartrate, 28% (w/v) PEG 3350, 10% (v/v) 2.0 M NDSB-221 |
