6ZYF

Notum_Ghrelin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92819
Spacegroup name	P 21 21 2
Unit cell lengths	75.418, 184.072, 61.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.040 - 2.190
R-factor	0.232
Rwork	0.231
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uzq
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.040	2.230
High resolution limit [Å]	2.190	2.190
Number of reflections	45353	2207
<I/σ(I)>	10.3
Completeness [%]	100.0	98.7
Redundancy	6.1
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	296	0.1 M Hepes pH 7.0 0.1 M KCl 15% PEG MME 5000