6ZYB
Sarcin-Ricin Loop RNA from Ecoli with a C2667-2'-OCF3 modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 43 |
| Unit cell lengths | 29.500, 29.500, 77.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.500 - 0.900 |
| R-factor | 0.1319 |
| Rwork | 0.131 |
| R-free | 0.14940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dvz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.552 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 0.920 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Number of reflections | 96381 | 3626 |
| <I/σ(I)> | 45 | |
| Completeness [%] | 100.0 | |
| Redundancy | 39.1 | |
| CC(1/2) | 1.000 | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3.2 M ammonium sulfate, 50 mM potassium MOPS, pH 7.0, 10 mM magnesium chloride, 10 mM manganese chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
