6ZXV
Structure of Archaeoglobus fulgidus Trm11 m2G10 tRNA methyltransferase enzyme bound to sinefungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.980096 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.553, 74.172, 112.894 |
| Unit cell angles | 90.00, 93.51, 90.00 |
Refinement procedure
| Resolution | 21.680 - 1.880 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.113 | 1.103 |
| Number of reflections | 54554 | 8304 |
| <I/σ(I)> | 10.2 | 1.2 |
| Completeness [%] | 98.2 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.996 | 0.585 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 0.1 M Hepes pH 7, 0.8 M KNaTartrate |
