Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.991, 74.138, 75.793 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.000 - 1.900 |
| R-factor | 0.2149 |
| Rwork | 0.213 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lar |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.364 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.070 | 41.070 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.087 | 0.051 | 1.253 |
| Rmeas | 0.094 | 0.056 | 1.349 |
| Rpim | 0.034 | 0.021 | 0.494 |
| Total number of observations | 2116 | 13403 | |
| Number of reflections | 29401 | 326 | 1847 |
| <I/σ(I)> | 9.1 | 23.7 | 1 |
| Completeness [%] | 99.6 | 99.3 | 99.3 |
| Redundancy | 7.5 | 6.5 | 7.3 |
| CC(1/2) | 0.998 | 0.997 | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 278 | 15% MMW PegSmear 0.1 M MES |
