6ZUS

Crystal structure of the effector Ecp11-1 from Fulvia fulva

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9734
Spacegroup name	P 21 21 21
Unit cell lengths	40.640, 53.640, 86.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.550 - 1.620
R-factor	0.1848
Rwork	0.184
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zuq
RMSD bond length	0.014
RMSD bond angle	1.807
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.550	1.720
High resolution limit [Å]	1.620	1.620
Rmeas	0.102	0.142
Number of reflections	46421	7420
<I/σ(I)>	10.56	0.86
Completeness [%]	99.7
Redundancy	7
CC(1/2)	0.998	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	21% PEG550MME, 10mM Zinc sulfate, 0.1 MES pH6.5, 15% glycerol