6ZUQ

Crystal structure of the effector Ecp11-1 from Fulvia fulva

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.28194
Spacegroup name	P 21 21 21
Unit cell lengths	40.760, 53.630, 86.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.130 - 1.940
R-factor	0.2007
Rwork	0.200
R-free	0.21210
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.816
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2 (2.0.203)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.133	43.133	2.060
High resolution limit [Å]	1.940	5.770	1.940
Rmerge	0.112	0.055	1.319
Rmeas	0.121	0.059	1.435
Total number of observations	184783
Number of reflections	26981	1023	4248
<I/σ(I)>	10.24	31.45	1.13
Completeness [%]	99.5	99.9	97.1
Redundancy	6.849	7.334	6.407
CC(1/2)	0.997	0.999	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	21% PEG550MME, 0.01M zinc sulfate, 0.09M MES pH6.5, 15% glycerol