6ZUF
Urea-based Foldamer Inhibitor chimera C2 in complex with ASF1 Histone chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.730, 51.280, 56.080 |
| Unit cell angles | 112.38, 104.87, 101.19 |
Refinement procedure
| Resolution | 48.000 - 1.798 |
| R-factor | 0.1862 |
| Rwork | 0.185 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i32 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.291 | 1.910 |
| High resolution limit [Å] | 1.798 | 1.800 |
| Rmerge | 0.084 | 0.660 |
| Number of reflections | 31049 | 3697 |
| <I/σ(I)> | 9.4 | 2.14 |
| Completeness [%] | 96.1 | 70.5 |
| Redundancy | 3.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 100 mM Sodium Citrate pH4.2, 300 mM LiSO4, 26% PEG3350 |
