6ZT7

X-ray structure of mutated arabinofuranosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.969
Spacegroup name	P 65 2 2
Unit cell lengths	156.790, 156.790, 378.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	135.790 - 1.850
R-factor	0.1443
Rwork	0.144
R-free	0.16610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vrq
RMSD bond length	0.024
RMSD bond angle	2.162
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	135.790	1.900
High resolution limit [Å]	1.850	1.850
Number of reflections	231584	16556
<I/σ(I)>	14.17
Completeness [%]	99.7	97.5
Redundancy	19.02
CC(1/2)	0.998	0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.2 M ammonium acetate, 0.1 M Bis-tris pH 5.5, 45% (v/v) MPD