6ZSU
Structure of crocagin biosynthetic protein CgnE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97797 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.400, 75.340, 112.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.060 - 2.000 |
| R-factor | 0.1585 |
| Rwork | 0.156 |
| R-free | 0.20180 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.909 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.13_2998) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.060 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.117 | 0.409 |
| Number of reflections | 41166 | 2997 |
| <I/σ(I)> | 16.9 | 7 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200 mM MgCl2, 100 mM Tris pH 8.5 and 20% PEG 8000 |
