6ZSH

The mechanism of activation of the actin binding protein EHBP1 by Rab8 family members

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000010
Spacegroup name	P 1 21 1
Unit cell lengths	54.060, 48.190, 100.300
Unit cell angles	90.00, 97.48, 90.00

Refinement procedure

Resolution	44.820 - 2.200
R-factor	0.1796
Rwork	0.177
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d89
RMSD bond length	0.015
RMSD bond angle	1.595
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.820	44.820	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.123	0.034	1.462
Rmeas	0.134	0.037	1.587
Total number of observations	173618
Number of reflections	26307	317	1939
<I/σ(I)>	11.15	31.39	2.04
Completeness [%]	99.8	96.9	99.9
Redundancy	6.6	5.486	6.74
CC(1/2)	0.998	0.999	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M di-Ammonium hydrogen citrate 20% (w/v) PEG 3350