6ZQR
Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1 with GlcNAc ligand bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 4 |
Unit cell lengths | 113.600, 113.600, 44.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.800 - 1.930 |
R-factor | 0.1683 |
Rwork | 0.167 |
R-free | 0.19120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 4M7H |
RMSD bond length | 0.007 |
RMSD bond angle | 1.409 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.31) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.860 | 56.800 | 1.980 |
High resolution limit [Å] | 1.930 | 9.050 | 1.930 |
Rmerge | 0.073 | 0.026 | 0.379 |
Rmeas | 0.086 | 0.031 | 0.453 |
Rpim | 0.044 | 0.015 | 0.243 |
Total number of observations | 1817 | 8913 | |
Number of reflections | 42095 | 463 | 2759 |
<I/σ(I)> | 12.4 | 29.7 | 3 |
Completeness [%] | 98.7 | 99.3 | 98.5 |
Redundancy | 3.5 | 3.9 | 3.2 |
CC(1/2) | 0.997 | 0.998 | 0.796 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.5 M Ammonium Sulphate, 7% Dioxane, 0.1 M MES pH 6.5 |