6ZPS

Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 3 Collected at 2.75 A

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I23
Synchrotron site	Diamond
Beamline	I23
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-12-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	2.755
Spacegroup name	P 43 21 2
Unit cell lengths	84.171, 84.171, 257.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.128 - 1.795
Rwork	0.144
R-free	0.17840
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.792
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	257.350	1.800
High resolution limit [Å]	1.790	1.790
Rpim	0.012	0.142
Number of reflections	74070	4624
<I/σ(I)>	35.1	5.7
Completeness [%]	84.4	90.5
Redundancy	70.2	21.5
CC(1/2)	0.999	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10 mg/mL MgGH51 in 10 mM NaOAc pH 5.5, 100 mM NaCl mixed 2:1 with 1.8 M (NH4)2SO4, 0.1 M NaOAc, pH 5-6, 35% glycerol