6ZPP
g7941: a virulence factor from Drechmaria coniospora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.127, 31.200, 59.316 |
| Unit cell angles | 90.00, 91.96, 90.00 |
Refinement procedure
| Resolution | 29.640 - 1.500 |
| R-factor | 0.1862 |
| Rwork | 0.185 |
| R-free | 0.20920 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.281 | 29.641 | 1.580 |
| High resolution limit [Å] | 1.500 | 4.740 | 1.500 |
| Rmerge | 0.115 | 0.484 | |
| Rmeas | 0.169 | 0.151 | 0.608 |
| Rpim | 0.065 | 0.060 | 0.234 |
| Total number of observations | 175009 | 5710 | 24875 |
| Number of reflections | 26120 | 888 | 3726 |
| <I/σ(I)> | 7.4 | 12.9 | 2.2 |
| Completeness [%] | 99.7 | 99.3 | 98.5 |
| Redundancy | 6.7 | 6.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05M Na Cacodylate pH5.0-6.0 0.05M Na Acetate 20-30% PEG-5000mme |
