6ZPN

Crystal structure of Chaetomium thermophilum Raptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999989
Spacegroup name	P 41 21 2
Unit cell lengths	183.306, 183.306, 271.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.610 - 3.500
R-factor	0.2288
Rwork	0.228
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ef5
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.970	3.600
High resolution limit [Å]	3.500	3.500
Number of reflections	58884	4557
<I/σ(I)>	5	0.8
Completeness [%]	99.9	100
Redundancy	12.8	13.2
CC(1/2)	0.989	0.367

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		303.15	0.1 M Bis-Tris pH 5.2-5.3 1.75 M NaCl 12 mM CaCl2