6ZPM
Crystal structure of the unconventional kinetochore protein Trypanosoma cruzi KKT4 coiled coil domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-22 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.620, 25.310, 136.880 |
Unit cell angles | 90.00, 96.81, 90.00 |
Refinement procedure
Resolution | 67.960 - 1.900 |
R-factor | 0.2429 |
Rwork | 0.241 |
R-free | 0.25830 |
Structure solution method | AB INITIO PHASING |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.960 | 3.880 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.095 | |
Rmeas | 0.103 | |
Rpim | 0.040 | |
Number of reflections | 16787 | 1452 |
<I/σ(I)> | 6.1 | |
Completeness [%] | 90.4 | |
Redundancy | 6.1 | |
CC(1/2) | 0.998 | 0.274 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | HEPES, sodium chloride, MOPSO, Bis-Tris, DL-Arginine hydrochloride, DL- Threonine, DL-Histidine monohydrochloride monohydrate, DL-5-Hydroxylysine hydrochloride, trans-4-hydroxy-L-proline, PEG 8000, 1,5- Pentanediol, TCEP |