6ZP6
Yeast 20S proteasome in complex with glidobactin-like natural product HB334
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.720, 301.640, 144.360 |
| Unit cell angles | 90.00, 113.08, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.1758 |
| Rwork | 0.174 |
| R-free | 0.20220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cz4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.061 | 0.543 |
| Number of reflections | 255091 | 25704 |
| <I/σ(I)> | 13.36 | 2.62 |
| Completeness [%] | 97.5 | 98.4 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |
