6ZOT

Crystal structure of YTHDF3 YTH domain in complex with m6A RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-02
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 4 21 2
Unit cell lengths	100.750, 100.750, 72.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.060 - 2.700
R-factor	0.2182
Rwork	0.215
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rci
RMSD bond length	0.011
RMSD bond angle	1.444
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.060	2.860
High resolution limit [Å]	2.700	2.700
Number of reflections	10844	1696
<I/σ(I)>	7.25
Completeness [%]	99.8
Redundancy	14.95
CC(1/2)	0.997	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.1M Tris pH8, 0.2M NaCl