6ZOE
AcrB-F563A symmetric T protomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 226.609, 226.609, 226.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.260 - 2.850 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jmn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.260 | 46.260 | 2.950 |
| High resolution limit [Å] | 2.850 | 11.040 | 2.850 |
| Rmerge | 0.195 | 0.039 | 2.022 |
| Rmeas | 0.196 | 0.040 | 2.031 |
| Rpim | 0.019 | 0.004 | 0.197 |
| Total number of observations | 4769509 | ||
| Number of reflections | 45123 | 826 | 4391 |
| <I/σ(I)> | 28.8 | 4.3 | |
| Completeness [%] | 100.0 | 98.7 | 100 |
| Redundancy | 105.7 | 99.4 | 106 |
| CC(1/2) | 1.000 | 1.000 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M sodium acetate, pH 5.5 and 12% PEG400 |
