6ZNP
Crystal Structure of DUF1998 helicase MrfA bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-31 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 140.332, 140.332, 212.106 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.320 - 3.160 |
| R-factor | 0.2215 |
| Rwork | 0.219 |
| R-free | 0.26430 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.726 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.350 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Number of reflections | 37055 | 5841 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 13.5 | 14 |
| CC(1/2) | 0.993 | 0.411 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M tri-sodium citrate, 24 % (w/v) PEG 3350 |
