6ZNJ

MaeB full-length enzyme apoprotein form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97951
Spacegroup name	C 2 2 21
Unit cell lengths	81.705, 273.583, 308.232
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.772 - 3.700
Rwork	0.210
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cee 1td9
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	154.120	4.000
High resolution limit [Å]	3.700	3.700
Number of reflections	36296	1825
<I/σ(I)>	4.6	0.5
Completeness [%]	97.1
Redundancy	13
CC(1/2)	0.960	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	289	0.1M Tris pH 8.0 20% MPD