6ZN3

Plasmodium facliparum glideosome trimeric sub-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 43
Unit cell lengths	211.880, 211.880, 75.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.420 - 2.510
R-factor	0.20216
Rwork	0.200
R-free	0.23756
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jt4 4aom
RMSD bond length	0.009
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER (1.17.1.3660)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.420	2.604
High resolution limit [Å]	2.510	2.510
Rmerge	0.087	3.790
Rmeas	0.095	4.118
Rpim	0.037	1.602
Number of reflections	114354	911
<I/σ(I)>	12.69	0.55
Completeness [%]	83.5	7.97
Redundancy	6.7	6.6
CC(1/2)	0.998	0.111

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	PEG 8000, ethylene glycol, di-ethyleneglycol, tri-ethyleneglycol, tetra-ethyleneglycol, penta-ethyleneglycol, imidazole, MES