6ZMQ
Cytochrome c Heme Lyase CcmF
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.720, 128.270, 134.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.150 - 2.670 |
R-factor | 0.2726 |
Rwork | 0.271 |
R-free | 0.30850 |
Structure solution method | MAD |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.980 | 3.060 |
High resolution limit [Å] | 2.670 | 2.670 |
Number of reflections | 15589 | 779 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 91.4 | 58.9 |
Redundancy | 26.8 | 23.5 |
CC(1/2) | 1.000 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 18-19 % (v/v) low molecular weight polyethylene glycols (PEG 400, PEG 550 MME, PEG 600, PEG 1000), 0.1 M BisTris pH 6.5, 150 mM MgCl2, 4 % (v/v) trifluorethanol |