6ZMP

Crystal structure of Chaetomium thermophilum Naa20 in complex with a bisubstrate analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976247
Spacegroup name	P 1 21 1
Unit cell lengths	46.150, 114.453, 47.416
Unit cell angles	90.00, 90.19, 90.00

Refinement procedure

Resolution	47.420 - 1.570
R-factor	0.1731
Rwork	0.171
R-free	0.20530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k18
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.420	1.600
High resolution limit [Å]	1.570	1.570
Rmerge	0.087	1.306
Rpim	0.056	0.817
Number of reflections	67562	3320
<I/σ(I)>	8.6
Completeness [%]	98.7	98.4
Redundancy	6.6	6.9
CC(1/2)	0.998	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	CtNaa20 was concentrated to 20 mg/ml and mixed in a 1:3 molar ratio with CoA-Ac-MDEL. The crystallization drops contained 200 nl protein solution and 200 nl precipitant solution (15 % (v/v) propanol, 0.2 M ammonium acetate and 0.1 M TRIS pH 8.5).