6ZMN

Crystal structure of the Smad3-Smad5 MH1 domain chimera bound to the GGCGC site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	54.502, 73.416, 111.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.000 - 2.333
R-factor	0.212
Rwork	0.210
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5od6
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.940	2.640
High resolution limit [Å]	2.370	2.370
Rpim		0.531
Number of reflections	12973	649
<I/σ(I)>	8.9
Completeness [%]	91.8	57.9
Redundancy	6.6	2.9
CC(1/2)	0.998	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% PEG 3350, 0.2 M sodium acetate