6ZMJ

Crystal structure of human GFAT-1 R203H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-04-28
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	152.766, 152.766, 164.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.653 - 2.774
R-factor	0.2021
Rwork	0.201
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r4e
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	PHENIX (dev_2499)
Refinement software	PHENIX (dev_2499)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.653	2.873
High resolution limit [Å]	2.774	2.774
Number of reflections	49792	4773
<I/σ(I)>	14.05
Completeness [%]	99.8
Redundancy	13.3
CC(1/2)	0.997	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 8.75, 0.2 M Potassium sodium tartrae, 20 % PEG3350