6ZLK
Equilibrium Structure of UDP-Glucuronic acid 4-epimerase from Bacillus cereus in complex with UDP-Glucuronic acid/UDP-Galacturonic acid and NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.737, 124.349, 98.364 |
| Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
| Resolution | 49.370 - 1.500 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u4q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.722 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.370 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 190286 | 14125 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.990 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 200 mM potassium acetate, 14-24% PEG3350 |
