6ZLH

the structure of glutamate transporter homologue GltTk in complex with the photo switchable compound (trans)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 31 2 1
Unit cell lengths	116.790, 116.790, 310.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.913 - 2.800
R-factor	0.2195
Rwork	0.218
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e9s
Data reduction software	XSCALE
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.913	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	61276	4451
<I/σ(I)>	9.85	0.41
Completeness [%]	99.9	100
Redundancy	9.9
CC(1/2)	0.990	0.236

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277.15	23% PEG 400, 100 mM sodium acetate, 50 mM NaCl, 20 mM MgCl2