6ZIV
Crystal structure of a Beta-glucosidase from Alicyclobacillus acidiphilus
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9139 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.654, 91.644, 159.342 |
| Unit cell angles | 91.62, 90.42, 89.99 |
Refinement procedure
| Resolution | 46.550 - 1.950 |
| Rwork | 0.262 |
| R-free | 0.29630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | sequence |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 159.274 | 1.640 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.098 | 0.561 |
| Number of reflections | 367007 | 18349 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 91.1 | |
| Redundancy | 84.9 | |
| CC(1/2) | 0.996 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | condition H3 (0.1 M Bis-Tris pH 5.5 and 25% (w/v) PEG3350) from the JCSG screen (Molecular Dimensions) |
