6ZIF
The structure of a cytosolic copper storage protein from Methylocystis sp. Strain Rockwell (ATCC 49242)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96878 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.760, 60.760, 231.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.770 - 2.200 |
| R-factor | 0.21362 |
| Rwork | 0.211 |
| R-free | 0.27036 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lmf |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.772 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.174 | |
| Rmeas | 0.190 | |
| Rpim | 0.074 | |
| Number of reflections | 22975 | 1957 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.3 | |
| Redundancy | 11.7 | 12.3 |
| CC(1/2) | 0.997 | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 200 mM MgCl2, 100 mM Hepes pH 7.5 and 25% PEG 3350 |
