6ZGT

Crystal structure of a MFS transporter with bound 2-naphthoic acid at 2.39 Angstroem resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	102.534, 199.623, 61.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.962 - 2.230
R-factor	0.2164
Rwork	0.215
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g9x
Data reduction software	XDS
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14_3228)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.660	49.660	2.350
High resolution limit [Å]	2.230	7.050	2.230
Rmerge	0.139	0.094
Rmeas	0.140	0.095	75.194
Rpim	0.019	0.013	10.243
Total number of observations	3733279
Number of reflections	62970	2199	9044
<I/σ(I)>	28.9
Completeness [%]	100.0	99.7	99.9
Redundancy	59.3	47.7	52
CC(1/2)	0.998	0.997	0.251

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291.15	zinc bromide, HEPES, jeffamine ED-2003