6ZGB

glutamate transporter homologue Glttk in complex with a photo cage compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 32 2 1
Unit cell lengths	115.740, 115.740, 308.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.748 - 3.200
R-factor	0.234
Rwork	0.232
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dwy
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.280
High resolution limit [Å]	3.200	3.200
Number of reflections	40350	2923
<I/σ(I)>	9.16
Completeness [%]	99.7	100
Redundancy	7.58	7.83
CC(1/2)	0.998	0.145

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	27%PEG350MME, 100 mM Tris 8.0, 40 mM NaCl