6ZFW
X-ray structure of the soluble N-terminal domain of T. cruzi PEX-14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2015-12-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.82656 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.688, 117.382, 51.301 |
| Unit cell angles | 90.00, 109.23, 90.00 |
Refinement procedure
| Resolution | 48.440 - 1.580 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l87 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.202 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.440 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmeas | 0.688 | |
| Number of reflections | 101657 | 10617 |
| <I/σ(I)> | 9.5 | 1.35 |
| Completeness [%] | 94.2 | 92.9 |
| Redundancy | 1.73 | 1.7 |
| CC(1/2) | 0.998 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M Na acetate, 0.1 M Tris.HCl pH 8.5, 30%(w/v) PEG 4000 |
